Introduction: The study was conducted to evaluate the relationship between mother’s employment status and prevalence of risk behavior among adolescent students in Lalitpur district of Nepal. Method: This comparative study was conducted in year 2013 with selected 110 secondary level students of purposively selected 4 public schools of Lalitpur district, Nepal. A total 110 mothers [55 employed and 55 unemployed] of selected adolescents were considered under the study. Information was collected by using pre tested structured interview schedule and collected data were analyzed by using SPSS version 19. Results: Adolescent students had poor knowledge on STDs and prevalence of risk behavior is found among adolescents of both working and non working with no association between development of risk behavior and employment status of mothers. Conclusion: Maternal employment status is not the exclusive reason behind the development of unhealthy practices and development of risk behavior among adolescents. The study suggests the need of awareness about the changing nature of adolescent to provide psychological and emotional support to children.